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Image Search Results
Journal: Nature Communications
Article Title: Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
doi: 10.1038/s41467-021-25576-6
Figure Lengend Snippet: Stool samples collected from omnivorous donors and processed into a single standardized matrix by NIST was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Article Snippet:
Techniques: Concentration Assay, Control
Journal: Construction and Building Materials
Article Title: Smart reversible thermochromic mortar for improvement of energy efficiency in buildings
doi: 10.1016/j.conbuildmat.2018.07.246
Figure Lengend Snippet: Fig. 2. Reflectance spectra of the reversibly thermochromic pigment and mortar in the solar range.
Article Snippet: Optical characterization of the thermochromic pigment and mortar was performed by measuring the spectral
Techniques: